Colo-205(人結(jié)腸癌腫瘤細(xì)胞)
CBP60026
                       
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                | I. General information | |
| Synonyms: | Colo-205 | 
| Background: | This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. | 
| Species: | Homo sapiens, human | 
| Tissue: | colon; derived from metastatic site: ascites | 
| Disease: | Dukes' type D, colorectal adenocarcinoma | 
| Gender: | male, 70 years, Caucasian | 
| Morphology: | epithelial | 
| Growth Mode: | mixed, adherent and suspension | 
| DNA Profile: | Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15 Our Cell Line Authentication Service  | 
		
| Culture Medium: | 
				 RPMI-1640+10%FBS Colo-205完全培養(yǎng)基,# CBP60026M We strongly suggest to purchase the complete medium from us. 
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| Cryopreservation medium: | 90%FBS+10%DMSO | 
| Genes Expressed: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10),The cells are positive for keratin by immunoperoxidase staining. | 
| Cellular Products: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10) | 
| Tumor Formation: | Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) | 
| Comments: | The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224). The cells are CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. For more information, please contact us (4008-750-250).  |