HVEM/NFκB-Luc/Jurkat
CBP74013
                       
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                | I. Background | |
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				       皰疹病毒進入介質(zhì)(HVEM),也稱為腫瘤壞死因子受體超家族成員 14 (TNFRSF14),是 TNF 受體(腫瘤壞死因子)超家族的人細胞表面受體。HVEM 在多種 免疫細胞中表達,包括 T 細胞、B 細胞、自然殺傷細胞、樹突狀細胞和內(nèi)皮細胞。它還通 過在黑色素瘤細胞上的頻繁表達參與腫瘤的免疫逃避。  | 
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| II. Description | |
      HVEM NFκB-Luc Jurkat 報告基因藥靶模型很好的模擬了體內(nèi) HVEM 的信號轉(zhuǎn)導(dǎo)過程, 原理見下圖所示。![]() Figure 1. HVEM NFκB-Luc Jurkat 細胞模型原理圖  | 
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| III. Introduction | |
| Host Cell: | Jurkat | 
| Expressed gene: | 
				 HVEM-NFκB-Luciferase  | 
		
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | 
| Synonym(s): | HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT | 
| Freeze Medium: | 90% FBS+10% DMSO | 
| Culture Medium: | RPMI-1640+10%FBS+800μg/ml hygromycin+1μg/ml puromycin | 
| Mycoplasma Testing: | Negative | 
| Storage: | Liquid nitrogen | 
| Application(s): | Functional(Report Gene) Assay | 
| IV. Description of Host Cell Line | |
| Organism: | Human | 
| Tissue: | Peripheral blood | 
| Disease: | Childhood T acute lymphoblastic leukemia | 
| Morphology: | Lymphoblast | 
| Growth Properties: | Suspension | 
| Ⅴ. Representative Data | |
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				 Figure 2. Recombinant Jurkat stably expressing human HVEM.  | 
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